This could also be the result of the loss of normal flora of the organ e.g. Lactobacillus in the vagina, which otherwise keeps the pathogens population under control. While on one hand, it is important to distinguish the normal flora from the pathogens, it is also imperative to identify the pathogen, in order to find the appropriate remedy for the infection. Several microbiological techniques are available for the detection, identification, and characterization of these microorganisms on the basis of their morphology and specific metabolic processes. Further theses microbes can also be identified on the basis of their specific antibiotic regime. The present experiment deals with deducing the cause of infection of the patients’ Lower GIT and vagina and therefore, samples are taken from the vaginal swabs and feces of the individual. This would help in determining the cause of the disease and hence providing the appropriate treatment.
The initial criteria for the identification of bacteria isolated from vaginal swab 1 (V1) were it being gram-positive. Gram’s staining helps differentiate bacteria on the basis of their cell wall characteristics (Ryan &. Ray, 2004). This result excluded all other genera of gram negative bacteria. Cell morphology of V1 being cocci, it is clearly distinguished from gram-negative bacilli, can be either of the following: Staphylococcus, Micrococcus, Streptococcus or Enterococcus. The colony morphology: round, raise, smooth, small and cream color is suggestive of it being either Staphylococci or streptococci. Mannitol salt agar is a selective and differential media for Staphylococci, also differentiating species of Staphylococcus which ferment or do not ferment mannitol. Since V1 is able to grow on Mannitol salt agar and is also able to ferment it, the bacteria V1 can safely be assumed to be Staphylococcus aureus. Since it is catalase positive the option .is reduced to two genera, Micrococcus and Staphylococcus, Streptococci are thus excluded.