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P7 16 2amino2hydroxymethylpropane1 3diol (Commonly Known As

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Chapter 7: Acid-base reactions
P7.16: 2-amino-2-hydroxymethylpropane-1,3-diol, (commonly known as ‘Tris’) and
imidazole are very commonly used as buffers in biochemistry and molecular biology
laboratories. You make two buffer solutions: One is 50 mM Tris at pH 7.0, the other
50 mM imidazole/imidazolium at pH 7.0 For each solution, calculate the concentration
of buffer molecules in the cationic protonation state.
OH
H
No
HaN-C
OH
N
OH
H
‘Tris’ buffer
imidazolium
PK, = 8.1
PK =7.0Science